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tes.Preparation of M. jannaschii Tyrosyl-tRNA Synthetase and its Evolved DerivativesE. coli BL21 (DE3) cells transformed with one of the above plasmids were grown to an OD600 of 0.5?.7 in 1 L Luria-Bertani (LB) medium. Isopropyl-?D-thiogalactoside (IPTG) was added to a final concentration of 1 mM and the cells were grown for additional 4? h at 37uC. Cells were harvested at 8,000 g for 10 min at 4u

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